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There is little doubt that the use of fine needle aspiration cytology (FNAC) in the diagnosis of thyroid nodules has markedly improved the management of patients. In recent years, however, new diagnostic techniques, such as immunocytochemistry, have emerged and have been applied to this field. A panel of monoclonal antibodies, including the monoclonal antibody (MAb) SP-1, has been applied to the study of a series of thyroid lesions: 20 follicular adenomas, 33 papillary carcinomas (PCa), 24 follicular variant of papillary carcinomas, 10 adenomas with PCa-like features (APCa), and 18 benign nodular lesions. The MAb SP-1 binds to a membrane glycoprotein (known as thyroglobulin) expressed in the normal thyroid cells and not in other tissues. The monoclonal antibody MAb-RT97, directed against the TSH receptor, labels thyroglobulin-negative cells in various types of follicular neoplasms and, in combination with SP-1, can be used to detect the presence of thyroid cancer cells. In the present study, we evaluated the performance of dual immunocytochemistry using the two antibodies SP-1 and MAb-RT97 in comparison with conventional morphology in the identification of thyroid carcinoma in FNAC samples. Our results show that dual immunocytochemistry is a useful technique in the differentiation between benign and malignant follicular neoplasms.Also known as BCG, bacille-calmette-guerin, and Can. Nos. 1 and 2.
Habitat
It is found in the environment in soil and underground animal reservoirs and in water and is generally used in a water treatment system or to treat municipal water sources.
History
BCG has been used therapeutically for many years, particularly in the treatment of superficial bladder cancer. 0b46394aab
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